Cinnamoyl-CoA reductase (CCR) is responsible for the first committed reaction in monolignol biosynthesis, which diverts phenylpropanoid-derived metabolites into the biosynthesis of lignin. To gain a better understanding of the lignin biosynthesis in wheat development, two cDNAs encoding CCR were identified from wheat ( Triticum aestivum L. cv. H4564). DNA sequence analyses indicated that the two cDNAs represent two classes of CCR. RT-PCR and Northern blot hybridization demonstrated that one of them, W-cr6, was expressed actively in stem and leaf tissue, the other one, W-cr19, was expressed in root and stem tissue. The results suggested that there are at least two genes encoded for CCR existing in wheat genome.
小麦中两个肉桂酰辅酶! 还原酶基因的分离和表达分析
蔺占兵 马庆虎* 麻密
（中国科学院植物研究所，北京100093）
摘要：肉桂酰辅酶A还原酶(CCR)负责催化木质素单体生物合成中最重要的代谢反应,它将类苯丙酸类代谢物转移到木质素的合成途径中.为了更好地了解木质素在小麦生长发育中的作用,从小麦(Triticum aestivum L. cv. H4564)中克隆了两个肉桂酰辅酶A还原酶的cDNA.相似性和进化关系的分析表明这两个cDNA片段分别属于不同的肉桂酰辅酶A还原酶,这两个cDNA片段分别命名为W-cr6和W-cr19.RT-PCR和Northern杂交结果证明,W-cr6基因主要在小麦的茎和叶中表达,W-cr19基因主要在根和茎中表达.上述结果表明在小麦的基因组中至少存在两类肉桂酰辅酶A还原酶基因.
关键词： 小麦；cDNA 克隆；肉桂酰辅酶% 还原酶；木质素
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