Abstract:Diverse genetic variation is of great importance for crop ecological adaption and improvement. Bread wheat (Triticum aestivum L.) is one of the most important staple food for human worldwide. Wheat coverage and production of Hebei province ranked third in China. However, systematically understanding for genetic diversity in this province is scare. Therefore, the objective of present study is to investigate the genetic diversity based on genome-wide SSR markers. The results showed that among the wheat accessions studied, genetic diversity in A genome, the seventh homoeologous group and chromosome 1A is relatively poor. From the historical view, genetic diversity of Hebei wheat accessions presented a slowly increasing trend. Furthermore, based on genetic similarity, these wheat accessions can be clustered into five groups, which did not reflect, to some extent, the diverse characters for Hebei wheat cultivars, but also the breeding behavior in different institutes of Hebei.