CuSO4 was added into Hoagland solution to imitate Cu2+ polluted water environment, and the roles of the two antioxidation systems in resisting Cu2+ stress in Alternanthera philoxeroides (Mart.) Griseb. was studied. The results showed that Cu2+pollution less than 70 mg/L in concentration could stimulate the accumulation of superoxide radical (O-·2), thus lead to lipid peroxidation and increase of membrane permeability. Superoxide dismutase (SOD) and peroxidase (POD) were both induced in high level and took important parts in antioxidation at higher concentration of Cu2+ and in middle and late phases of Cu2+ stress. Catalase (CAT) had little effect. Glutathione peroxidase (GSH-Px) was induced in lower level and showed its function in lower Cu2+ concentration and in early phase. Ascorbic acid (AsA) could also function at higher Cu2+ concentration by transition from reduced state to oxidized state.
水花生抗氧化系统在抵御Cu2+ 胁迫中的作用
周长芳* 吴国荣 施国新 陆长梅 顾龚平 宰学明 魏锦城
（南京师范大学生命科学学院，南京210097）
关键词： 水花生；抗氧化系统；Cu2+ 胁迫
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