The high molecular weight (HMW) glutenin subunits and their coding genes from Aegilops umbellulata Zhuk. (UU , 2n = 2x = 14) were characterized using SDS PAGE analysis and molecular approaches. SDS PAGE analysis showed that the 1Ux subunits from four different accessions possessed electrophoretic mobilities close to, or slower than, that displayed by the 1Dx2.2 subunit of common wheat.The electrophoretic mobilities of the 1Uy subunits were generally similar to those shown by the 1Dy subunits of common wheat. The complete open reading frames of the 1Ux and 1Uy genes were amplified by PCR and subsequently cloned and sequenced. Amino acid sequence comparisons suggested that the primary structure of the 1Ux and 1Uy subunits were identical to that of published HMW glutenin subunits from related species. Phylogenetic analysis indicated that the HMW glutenin subunits of Ae. umbellulata were most closely related to those encoded by the D genome of Triticeae.
小伞山羊草高分子量麦谷蛋白亚基及其基因的鉴定
刘志杰 张学民 万永芳 刘坤凡 王道文*
（中国科学院遗传与发育生物学研究所植物细胞与染色体工程国家重点实验室，北京100101）
摘要： 运用SDS-PAGE和分子克隆技术,对小伞山羊草(Aegilops umbellulata,UU, 2n = 2x = 14)的高分子量麦谷蛋白亚基(1Ux, 1Uy)及其编码基因进行了鉴定.SDS-PAGE分析表明小伞山羊草不同基因型中的1Ux的电泳迁移率接近或慢于普通小麦1Dx2.2亚基的电泳迁移率,1Uy亚基的电泳迁移率一般接近或慢于普通小麦的1Dy类亚基.采用PCR扩增技术获得了1Ux和1Uy亚基编码基因的全长编码区,并对一个1Uy基因的全长编码区进行了全序列测定.对推导的氨基酸序列进行比较发现1Ux和1Uy亚基具有与来自于其他物种的高分子量麦谷蛋白亚基一致的一级结构,聚类分析显示1Ux和1Uy亚基与D基因组编码的高分子量麦谷蛋白亚基在起源和进化上具有较高的相似性.
关键词： 高分子量麦谷蛋白亚基；山羊草属；小伞山羊草
通讯作者。E-mail::<dwwang @ genetics.ac.cn>.
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