A simple and rapid new method is presented to determine the CO2/O2 specificity factor for ribulose-1,5-bisphosphate(RuBP) carboxylase/oxygenase (Rubisco). In principle, the quantitative formula, which intrinsically resulted in error or enormous error in all reported methods, was improved to circumvent the problem. In practice, the complicated procedure with radiolabelling and chromatographic separation of 3-phosphoglycerate and 2-phosphoglycolate was substituted by one-step procedure without radiolabelling and chromatographic separation to decrease the random error. The effects of pH, temperature and ionic strength on accuracy of the data and the reliability of the calculation of the CO2/O2 specificity factor are also discussed.
一种简单快速测定Rubisco C02/O2特异性因子的方法
李国富1 陆巍2 李森1 公衍道1 张秀芳1 张荣铣2 周海梦1 赵南明1*
（1. 清华大学生物科学与技术系生物膜与膜生物工程国家重点实验室，北京100084；
2. 南京农业大学水稻研究所，南京210095）
摘要：提出了一种简单快速测定1,5-二磷酸核酮糖羧化/氧化酶CO2/O2特异性因子的方法.理论上改进了定量计算公式;操作上避免了使用放射性同位素标记以及层析分离3-磷酸甘油酸和2-磷酸乙醇酸的复杂程序,使测定过程一步完成,极大地减少了随机误差.讨论了实验数据(pH、温度、离子强度)的准确性对计算结果的影响.
关键词：1,5-二磷酸核酮糖羧化/ 氧化酶；CO2/02特异性因子；误差；准确性
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