Nine rice Oryza sativa L.） mutant lines lacking the seed storage glutelin α-2 subunit were obtained from the progenies of fertilized egg cells treated with N-methy-N-nitrosourea (MNU). The mutants could be classified into three types: the α-1 subunit increased type (α-1H/α-2L), decreased the β-2 subunit decreased type (β-2L/α-2L) and the α-3 subunit increased type (α-3H/α-2L) according to their SDS-PAGE profiles. Two-dimensional electrophoresis analysis revealed that all of the mutants lacked a polypeptide of pI 6.71/α-2, while new polypeptides of pI 6.50/α-1 and pI 6.90/α-3 formed in α-1H/α-2L and α-3H/α-2L mutants respectively. Although the β-2L/α-2L mutants did not form new polypeptide, their pI 8.74/β-2 polypeptide was also decreased, suggesting that the two polypeptides decreased in β-2L/α-2L mutants might derive from the same glutelin precursor. These mutant lines are very useful in studying genetic characterisation,the mechanism of genetic regulation on biosynthesis, gene function and proteomics of rice seed storage glutelin.
水稻种子贮藏谷蛋白的微细异质性
曲乐庆1 *魏晓丽1* 佐藤光2 小川雅广3 熊丸敏博2 贾旭1
（1. 中国科学院遗传研究所，北京100101；2. 日本国立九州大学农学部，福冈812-8581；
3. 日本山口县立大学生命科学部，山口753-8502）
摘要：利用灵敏的等点聚焦与SDS_PAGE结合的双向电泳分析方法,从水稻(Oryza sativa L.)种子贮藏谷蛋白中至少可以分离为13条酸性和19条碱性多肽.依据谷蛋白多肽的表现量推测,水稻谷蛋白主要由约6个主效基因控制.肽图谱与N_端氨基酸序列分析可清晰将谷蛋白酸性多肽分为两组.此两组恰好与谷蛋白GluA和GluB两个cDNA克隆组相吻合.
关键词： 水稻谷蛋白；双向电泳；N-端氨基酸序列
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