Abstract:Three restoring lines (Minghni 63, Ce 64 and Teqing) as female parents were crossed with herbicide-resistant transgenic cultivars (Bengal-Hul0 and Gulfmont) as male parents. Genetic analysis on generations of F1, F2 and BC1 indicated that the resistance to the herbicide (Liberty) was controlled by a single deminant nuclear gene. The resistance to the herbicide will work as a marker for the true hybrid from crossing with the male parent, but not for all other plants from either selfing, or intracrossing in female parent population. After the application of the herbicide, all plants except the true hybrid were eliminated. With the aid of this technology, the strict requirement of complete sterility for male sterile hne in crops can be reduced. It will be beneficial not only for breeding new excellent male sterile lines, but also for commercializing chemically induced male sterile system in crops.