---

期 刊 ：植物遗传资源学报 2015年 16卷 6期

关键词：苜蓿雄性不育系；SSR标记；牧草产量配合力；遗传距离；杂种优势；

Keywords:Alfalfa Male Sterile Lines, SSR Markers, Forage Yields Combining Ability, Genetic Distance, Heterosis,

摘 要 ：利用SSR标记技术对40份苜蓿材料（6个雄性不育株系和34个苜蓿品种）的遗传距离进行分析，并利用其中的6个不育株系与14个苜蓿品种测交，进一步对遗传距离(GD)、产量配合力与杂种优势效应进行相关性分析。结果表明，25对SSR引物共扩增出189条谱带，其中多态性条带136条，平均多态性位点百分率为69.23%；40份苜蓿材料的遗传距离为0.1818～0.9091，平均0.4544；各亲本一般配合力及GD均与杂种优势效应存在显著正相关，其中亲本一般配合力与杂种优势的相关性要高于GD.因此，仅以SSR遗传距离还不足以准确的组配强优势组合，需结合各性状配合力的分析，以充分发挥杂种优势效应。

Abstract:Heterotic grouping of 40 Alfalfa materials(six male sterile lines and 34 alfalfa breeding materials) genetic distance were analyzed by SSR markers, and using the six sterility strain and 14 alfalfa varieties test cross lines, in further to analysis for genetic distance (GD), yield combining ability and heterosis effect. The results show that, article 25 for SSR primers amplification out 189 band, including 136 polymorphic bands, the average polymorphism loci percentage was 69.23%. The genetic distance of 40 copies of alfalfa materials was 0.1818-0.9091, an average of 0.4544. Each parent effect of general combining ability were significant positive correlation with alfalfa heterosis, and GD ware also that, the correlation between general combining ability and heterosis was higher than GD and heterosis. Therefore, SSR genetic distance was not enough to assemble strong hetrosis combination, should be based on the character analysis of combining ability.

---