Abstract:On the basis of the reported location of the Pm6 gene, 36 homoeologous group-2 specific probes were selected to detect polymorphism between wheat/Triticum timopheevi Zhuk. Pm6 introgression lines and their recurrent parent "Prins". Two Pm6 introgression lines IGV1-456, IGV1-458 were distinguished from the others. Nineteen long ann specific and six short ann specific probes detected the RFLPs between resistant IGV1456, IGV1-458 and susceptible control "Prins", indicated that the introgressed T. timopheevi 2G chromatin involve beth arms spanning across the centromere between markers Xcdo405 and Xbcd135. Only 6 of the nineteen long ann specific probes and two additional probes－BCD292, CDO678 showed RFLPs between chromosome 2B of "Prins" and IGV1-463. This means that the introgressed T. timopheevi segment in IGV1- 463 with breakpoints between markers Xbcd307 and Xcdo678 is smaller than those detected in IGV1-456 and IGV1-458. Two of the six long arm specific probes PSR934, BCD135 detected polymorphism between IGV1- 464 and "Pr ins", and only one clone BCD135 revealed RFLPs between IGV1-465 and "Pr ins", which indicated that the introgressed segments in these two lines are smaller than those in others. As the introgressed segments in all the introgression lines bear the Pm6 gene, after comparison of the overlaps of the introgressed segments, it might be reasonable to map the gene Pm6 in the region of marker Xbcd135-2BL flanked 2BL.