Abstract:Hybridization between Oryza sativa L. ssp. indica and 0. sativa ssp. japonica could produce heterosis but various reproductive barriers existed. The barriers included embryo sac sterility, pollen sterility, anther indehiscence and non-synchronization of male and female gamete development in the same spikelet. A molecular linkage map comprising 137 markers was constructed based on 127 doubled haploid (DH) progenies from anther culture of Fl hybrid between an indica rice variety "Zhaiyeqing 8" and a japonica rice variety "Jingxi 17". The linkage map was employed to locate quantitative trait loci (QTL) for reproductive barriers in DH population and its derived two backcross populations by interval mapping. Ten QTLs were detected and located on chromosomes 1, 3,4,5,6,7,8,and 12 respectively. The positions of two QTLs located on chromosomes 3 and 12 were identical to the positions for distorted segregation detected in Fa population from the same cross. All the QTIs had additive effects in increasing sterility except for stj-6 that increased fertility.