Abstract:Peroxidase (PER), malate dehydrogenase (MDH), esterase (EST), alcohol dehydro-genase (ADH), acid (ACPH) and alkline (PHE) phosphatase zymogram phenotypes were analysed in Set Ⅱ wheat-wheatgrass alien addition lines and their parents. A number of isozyme structural genes have been located onto the individual wheatgrass chromosomes in different addition lines, e.g., genes perl-agil and mdh-agi2 in line TAI-21, est-agi3 in TAI-22, est-agil in TAI-23. est-agi4 in TAI-24, and genes adhl-agil, acph-agi2 and phe-agil in TAI-27, respectively. These results, combined with certain morphological characters of the plants, suggest that the alien addition lines TAI-21, TAI-22, TAI-23, TAI-24, TAI-25, TAI-26 and TAI-27 carry the wheatgrass chromosomes which are, to a certain extent, homoeologous to the wheat chromosomes belonging to homoeologous groups 1, 7, 3, 6, 2, 5 aud 4 respectively.