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作 者 ：朱凡,田大伦,闫文德,王光军,梁小翠,郑威

期 刊 ：生态学报 2008年 28卷 9期 页码：4195~4202

关键词：多环芳烃；土壤酶活性；磷酸酶；过氧化氢酶；多酚氧化酶；

Keywords:soil enzyme activity, phosphatase, hydrogen peroxidase, polyphenol oxidase,

摘 要 ：多环芳烃（PAHs）是一类广泛存在于环境中的有机污染物, 由于其致癌性和致突变性而受到广泛关注。采用盆栽实验，在3种多环芳烃污染水平（重度L3 ）、（中度L2）、（轻度L1）下，研究了樟树（Cinnamomum camphora）、广玉兰（Magnolia grandiflora）、栾树（Koelreuteria bipinnata）、马褂木（Liriodendron chinense）南方4种绿化树种的土壤酶活性在6个月后的响应差异。结果表明：在不同PAHs污染水平下，磷酸酶活性表现为L2＞L3＞ L1；多酚氧化酶和过氧化氢酶的趋势一致，表现为L3＞L1＞ L2。土壤酶活性在4个树种间有明显差异，在L1污染水平下，各土壤酶活性表现为马褂木＞广玉兰＞樟树＞栾树；在L2污染水平下，各土壤酶活性表现为栾树＞马褂木＞樟树＞广玉兰；而在L3污染水平下，表现为马褂木＞樟树＞广玉兰＞栾树。不同PAHs水平下的多酚氧化酶活性呈极显著差异(P＜0.01)，过氧化氢酶活性呈显著差异(P＜0.05)，而磷酸酶活性变化率受污染物浓度影响不显著。此外，土壤酶活性与微生物相关性不显著。土壤过氧化氢酶和多酚氧化酶可以作为土壤污染程度的评价指标。

Abstract:Polycyclic aromatic hydrocarbons (PAHs) are a kind of organic pollutants which exist in environments chronically, they are of particular concern because some are potent toxins and some can be highly persistent in the environment, also pose a carcinogenics risk to humans. The aim of this trial was to quantify the responses of soil enzyme activity during the phytoremediation of polycyclic aromatic hydrocarbons(PAHs) under laboratory control. In the experiments 4 tree species including Cinnamomum camphora, Magnolia grandiflora, Koelreuteria bipinnata, Liriodendron chinense, from subtropical China were elected and planted separatedly in the pots in which soils were treated with diesel oil to three concentration levels of PAHs(L1﹤L2﹤L3). Throughout the 180-d experiment of soil phosphatase, polyphenol oxidase and hydrogen peroxidase activity were monitored. Under different concentration of PAHs, the order of phosphatase activity was L2>L3> L1, the order of polyphenol oxidase and hydrogen peroxidase activity was L3> L1> L2. There are difference in enzymatic activities among 4 tree species. Treated with L1, the order of 4 tree species was Liriodendron chinense﹥Magnolia grandiflora﹥Cinnamomum camphora﹥Koelreuteria bipinnata；treated with L2，the order of 4 tree species was Koelreuteria bipinnata﹥Liriodendron chinense﹥Cinnamomum camphora﹥Magnolia grandiflora；treated with L3，the order of 4 tree species Liriodendron chinense﹥Cinnamomum camphora﹥Magnolia grandiflora﹥Koelreuteria bipinnata. The change ratio of polyphenol oxidase and hydrogen peroxidase activity showed significant difference with PAHs concentration. The soil enzymes showed few correlation with soil microbes. The activities of soil polyphenol oxidase and hydrogen peroxidase can be regarded as indexes to assess PAHs contaminated soil.

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