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作 者 ：蔡爱军,马子川*,刘敬泽*

期 刊 ：生态学报 2008年 28卷 11期 页码：5684~5690

关键词：LSZ；α-MnO2；吸附；脱附；

Keywords:lysozyme, α-MnO2, adsorption, desorption,

摘 要 ：研究了溶菌酶(LSZ)与α-MnO2的界面吸附作用及其影响因素。结果表明，体系pH值是影响LSZ在α-MnO2表面吸附、脱附的重要因素。在pH3.8～10.8范围内，LSZ的吸附率随着体系pH值的升高而大致呈上升趋势。在较低pH值范围内(pH<7.1)，LSZ的脱附率随pH的升高增幅较大；在较高pH值范围内(pH>7.1)，LSZ的脱附率则随pH的升高而略微下降。但是，LSZ自α-MnO2表面的脱附率在各pH值下均处于较低水平，吸附反应具有较高的不可逆性。LSZ在α-MnO2表面的吸附率随离子强度的增大而增大，然而Na+、Ca2+和Mg2+3种离子对LSZ吸附率的影响没有差别。LSZ的等温吸附曲线符合Langmuir吸附方程式，形状呈L型。FTIR进一步证明，α-MnO2表面的确有LSZ分子吸附；吸附态LSZ分子的空间构象发生了改变。

Abstract:In this work, the adsorption of lysozyme（LSZ）on the α-MnO2 and by which factors this process is affected were investigated. The results showed that the system pH value was a most important factor affecting adsorption and desorption of LSZ. In the pH range of 3.8 to 10.8, the adsorption percentage of LSZ on α-MnO2 was increased as pH value raised. The desorption percentage of lysozyme usually increased at low pH condition（pH<7.1）but slightly decreased at high pH condition(pH>7.1). However, the desorption percentage was generally low at all pH values tested, indicating the irreversibility of protein adsorption to the surface of α-MnO2. We also found increasing the ionic strength might increase the LSZ adsorption percentage,but there was no difference on effects of Na+、Ca2+ and Mg2+ on this adsorption. The LSZ adsorption isotherm belonged to the L type, and was fitted well by the Langmuir model. FTIR spectra further approved that LSZ was attached to the surface of mineral, and the spacial structure of attached protein was changed.

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