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作 者 ：马小龙,王芸,杨红梅,王纯利,毛培宏,金湘,常玮,房世杰,张评浒,娄恺*

期 刊 ：生态学报 2009年 29卷 7期 页码：3722~3728

关键词：泥火山；微生物多样性；非培养方法；系统发育；

Keywords:Mud volcano, Microbial diversity, Culture-independent method, Phylogeny,

摘 要 ：为了解新疆乌苏泥火山细菌多样性，从泥火山泥浆样品中直接提取总DNA，构建了含150个有效转化子的泥火山细菌16S rDNA基因文库,转化子经菌液PCR及HaeⅢ酶切后获得16个不同带型，克隆测序结果表明,其分属于16个不同的分类单元。一部分序列与已知细菌类群的16S rDNA序列相似性较高，归属变形菌门(Proteobacteria)，厚壁菌门(Firmicutes)，梭杆菌门(Fusobacteria)，放线菌门(Actinobacteria)；另外一部分序列与已知细菌类群的16S rDNA序列同源性较低,可能代表新的分类单位。研究结果显示,泥火山环境中微生物种群丰富,值得进一步研究。

Abstract:In this study, the mixed environmental genomic DNA was isolated directly from the mud volcano soil in Usu county of Xinjiang. The 16S rRNA genes were amplified with bacterial universal primers and cloned into the pGEM-T Easy Vector to construct a16S rRNA clone library．The total 150 clones were selected by amplified rDNA restriction analysis（ARDRA） using restriction enzymes Hae Ⅲ. Of these, 16 restriction fragment length polymorphism（RFLP）types were used for sequencing. A part of these sequences were closely related to the phylum: Proteobacteria，Firmicutes，Fusobacteria and Actinobacteria (>97% sequence similarity), while another portion showed less affiliation with known taxa (<97% sequence similarity) and might represent novel taxa. The results indicated that the mud volcano in Usu county of Xinjiang is rich in microbial species，which is worth further studying.

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