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作 者 ：张永军

期 刊 ：生态学报 2001年 21卷 12期 页码：2051~2056

关键词：转Bt基因棉花；挥发性它感物质；气质联机；

Keywords:transgenic Bt cotton, volatile allelochemics, GC-MS,

摘 要 ：应用顶空进样气质联机系统 ( Headspace-GC-MS)测定了转 Bt基因棉花及其对照亲本主要挥发性物质。结果表明棉花营养器官和繁殖器官的主要挥发性物质有差异 ,棉花植株现蕾期前 ,叶片中挥发性物质以 α-蒎烯为主 ,而现蕾后 ,蕾、花和铃中主要以β-月桂烯为主。研究初步发现 ,外源 Bt杀虫蛋白表达对棉花自身主要挥发性物质的合成不会造成不利影响。

Abstract:By using the headspace collection and GC-MS, the differences in composition and concentrations of volatile allelochemics between transgenic Bt cotton and their parental varieties during various developmental stages were compared and analyzed. The cotton varieties DP5415-NUCOTN33 B, Simian3-GK2 and CCRI16-CCRI30 of both the parental variety and Bt transgenic cotton, were planted in an experimental field of Institute of Plant Protection, CAAS. The following plant parts in cotyledon-, three-leaf-, seven-leaf-...

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