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作 者 ：胡俊杰， 石长青， 张勇， 王娟红， 常卫华

期 刊 ：核农学报 2016年 30卷 1期 页码：50-57

关键词：高山细毛羊；microRNA；高通量测序；GO注释；KEGG通路分析；

Keywords:alpine fine wool sheep, microRNA, high-throughput sequencing, GO annotation, KEGG pathway analysis,

摘 要 ：为丰富绵羊miRNA数据库和了解miRNA对哺乳动物卵巢的调控机理,应用高通量测序技术成功构建甘肃高山细毛羊性成熟期卵巢miRNA的cDNA文库,获得9 321 775 条clean reads,鉴定出267条绵羊新miRNAs序列,分析发现候选miRNA首位碱基对U和A具有偏向性。候选miRNA共预测到12 692条靶基因,GO注释表明,多达70%的基因与细胞及细胞间组分和元件有关,超过77%的基因参与了细胞过程,约78.5%的基因具有结合功能。KEGG通路分析显示约10.61%的基因与代谢通路有关。试验结果对研究绵羊卵巢miRNA具有一定的理论价值和现实意义。

Abstract:In order to rich miRNA database of Ovis aries and investigate regulation mechanism of miRNA on mammalian ovaries. Sexual maturity period ovarian miRNA cDNA library was successfully constructed by high-throughput sequencing methods, and 9 321 775 clean reads have been obtained. Novel miRNAs of 267 were identified from those clean reads and 12 692 target genes were predicted. Meanwhile,the first base pairs of candidate miRNAs have biased for U and A. With GO analysis, more than 70% of the genes were related to cell or intracellular part component ontology, more than 77% of the genes were involved in cellular processes and approximately 78.5% of the genes had binding functions. KEGG Pathway analysis showed that about 10.61% of the genes were committed to a metabolic pathway. The results will have certain theoretical guidance and practical significance for further research on miRNAs function of Ovis aries ovary.

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