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作 者 ：张华丽， 王涛， 宋利娜， 董爱香， 辛海波， 义鸣放， 赵金华

期 刊 ：核农学报 2016年 30卷 1期 页码：42-49

关键词：菊花；EST-SSR；万寿菊；可转移性；

Keywords:chrysanthemum morifolium, EST(expressed sequence tags)-SSR(simple sequence repeats), Tagetes erecta L., transferability,

摘 要 ：为开发菊花EST-SSR标记并用于万春菊资源分析,研究了菊花的EST-SSR特征、标记开发及其在万寿菊上的可转移性。结果表明,菊花EST-SSR出现频率为5.35%,平均每10.64 kb存在1个SSR位点。在这些SSR位点中,共有83种重复基元,2-6核苷酸重复类型分别为10、40、14、5和14种。以14个菊花品种DNA为模板,22对菊花EST-SSR引物中14对可以扩增到清晰且稳定的PCR产物,共检出 49 个位点,平均每对引物可扩增出3.5条多态性片段,平均多态性信息含量(PIC)为0.751。在14对有效引物中,8对可以在待检万寿菊样品中扩增到清晰稳定的条带,可转移率为57.14%。综上所述,菊花以三核苷酸重复类型占主导,六核苷酸重复类型高于其它植物种类,菊花EST-SSR引物多态性信息含量处于较高水平。本研究可为万寿菊分子标记辅助育种奠定科学基础。

Abstract:This study aims to characterize EST-SSR in Chrysanthemum morifolium, development SSR markers and clarify the transferability of them to Tagetes erecta. With these sequences downloaded from NCBI, SSR sites occurred with an occurrence frequency of 5.35% (1 per 10.64 Kb). Among the 83 kinds of repeat motifs, di-, tri-, te-, pe-, and he- nucleotides were 10, 40, 14, 5 and 14 types, respectively. Using 14 cultivars of Chrysanthemum morifolium as templates, 14 among 22 pairs of primers were effective. In this process, 49 polymorphism sites were detected with average values of 3.5 bands per primer pair and 0.751 of PIC. Furthermore, among 14 pairs of primers, clear and stable bands could be amplified by 8 primer pairs using Tagetes eracta as templates, with a transferability rate of 57.14%. Taken together, trinucleotide type was dominant in Chrysanthemum morifolium, and henucloetide types were more than these in other species. At the same time, these EST-SSRs contained higher PIC values. These primers from Chrysanthemun morifolium EST could be used as molecular markers of Tagetes eracta.

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