Abstract:In order to provide more selection of abiotic stress-inducible promoters for transgenic study in maize, and search those promoting exogenous genes appropriate expression only under stress conditions. The promoter (psbCBF6) of the ethylene-responsive element-binding protein gene (sbCBF6), homologous to the transcription factor gene DREB1B in rice, was cloned from maize based on bioinformatics analysis. After validation for its response to abiotic stresses by analysis for abiotic stress-responsive elements and real-time quantitative PCR, psbCBF6 was used to construct expression vector to drive the reporter gene GUS. This vector was used to transform maize calli by biolistic bombardment. The activity of the promoter psbCBF6 under the induction of different abiotic stresses and hormones was validated by the ratio of the GUS fluorescence value divided by the luciferase luminescence value. The results showed that the expression of the sbCBF6 gene was differential in responsive to different abiotic stresses. The psbCBF6 promoter is 1479 bp long, and contains various regulative elements related to abiotic stresses.The results of the study can improve a reference for the resistance of maize transformation.