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作 者 ：null

期 刊 ：核农学报 2009年 23卷 6期 页码：981-985

关键词：根瘤菌；微波诱导；生长素；耐药性；

Keywords:rhizobium, microwave mutagenesis, IAA, antibiotic resistance,

摘 要 ：为获得高效分泌IAA(吲哚乙酸Indoleacetic acid)，同时具有卡那霉素(Kanamycin)和氨苄青霉素(Ampicillin)双抗药性的根瘤菌突变株，以红豆草根瘤菌(Rhizobium Onobrychis vicilfolia)RS-1为原始菌株进行微波诱变处理，对微波诱变参数进行了优化，并以含抗生素平板筛选出卡那霉素和氨苄青霉素双抗药突变菌株，以多次传代的方式考察了突变菌株的遗传稳定性和生长素产量。结果显示，最优微波筛选参数为800W、6s，该参数下诱变获得5株高产生长素及双耐药突变株RSW-14、RSW-55、RSW-62、RSW-96和RSW-107。传代试验表明RSW-96是稳定的高产生长素、耐药突变株，表现在RSW-96传代6次后对80mg/L卡那霉素和300mg/L青霉素具有抗性，培养4d和24d的IAA产量比原始菌株提高了40.09%和50.15%，对单株生物量增加效果高于原始菌株25.58%，具有良好的促生效应。

Abstract:In order to obtain high IAA-producing rhizobium strain with antibiotic-resistant of Kanamycin and Ampicillin, the IAA-producing rhizobium strain RS-1 was irradiated by microwave. The mutagenesis parameters were optimized, and the genetic stability, IAA-producing potential and antibiotic resistance of the mutant were investigated by continuous subcultures. Results show that: 5 high IAA-producing strains with two antibiotic-resistant named RSW-14, RSW-55, RSW-62, RSW-96 and RSW-107 were obtained through the microwave irradiating (800W, 6s). Subculture test indicated that RSW-96 had relatively stable hereditary characteristics of IAA-producing capability and antibiotic resistance. It displayed mainly: Upon continuous subculture more than 6 generations, RSW-96 keep the ability of resistance to 300mg/L ampicillin and 80mg/L kanamycin, and the IAA-producing potential reaching 8.38mg/L (4d) and 50.75mg/L (24d) were 40.09% and 50.15% higher than the original strain, respectively. The ratio of promote the biomass increasing rate of RSW-96 were 25.58% higher than the original strain significantly (P＜0.05).

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