Abstract:Pollens of Sumian22 were irradiated by 60Coγ- rays at a dose of 20Gy，then fertilized to pistil and harvested seed. Three male-sterile lines were selected from M1 plants, their anther observed by paraffin slice technique. Although there were some different characteristics during the abortion anther development, the abortion was consistent：the abortion stage from the development periods of pollen mother cells to microspore， pollen mother cells, tapetum, middle layer cells and the shape of anther were affected, the results contained micronucleus and double nucleus, cytoplasm expansion during the period of meiosis, tapetum and middle layer cells were abnormal, and so on were abnormal.