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作 者 ：秦新民,熊军,阳晶,万珊,韦素玲

期 刊 ：热带亚热带植物学报 2008年 16卷 5期 页码：425~429

关键词：沙田柚；自交不亲和；抑制性消减杂交；cDNA文库；

Keywords:Citrus grandis var. shatinyu Hort, Self-incompatibility, Suppression subtractive hybridization (SSH), cDNA library,

摘 要 ：分别以沙田柚自交花柱cDNA为tester, 异交花柱cDNA为driver, 利用抑制性消减杂交技术构建了消减文库，文库的重组率高于95%，插入片段集中在100~500 bp之间，对文库部分克隆进行测序并与GenBank中的同源序列进行比较，发现了一些类似于S1、S9-RNase、激酶类等与自交不亲和相关的基因。

Abstract:A suppression subtractive hybridization library of Citrus grandis var. shatinyu Hort was constructed by using the cDNA of self-pollinated styles as the tester and cross-pollinated styles as the driver. In the constructed library, the rate of recombination was more than 95% and the size of inserts ranged from 100 bp to 500 bp. After the partial clones of the library were sequenced and compared with homologous sequences in GenBank Database, some genes related with self-incompatibility were obtained, such as S1, S9-RNase and kinase.

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