Abstract:A male sterile line of resynthsizing Brassica napus was discovered in the interspecific crossing between Brassica napus and B. rapa. The fertility segregation in brother-sister mating (BSM) lines and the hybrids F1, F2 lines from male sterile lines and B. napus cultivars were studied, which showed that the male sterile materialswere double recessive genic male sterile. Using self-crossing and test crossing of different lines in fertility segregated lines, 7 homozygous fertile lines (B1~B7) were screened out, and two genotypes (Ms1Ms1ms2ms2 and ms1ms1Ms2Ms2) were identified. These male sterile materials would play an important role in heterosis utilization and male sterile gene mapping.