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作 者 ：李国民,李忠超,许凯扬,文李

期 刊 ：热带亚热带植物学报 2008年 16卷 2期 页码：116~122

关键词：冬凌草；遗传多样性；ISSR；核心种质；

Keywords:Rabdosia rubescens (Henmsl.) Hara, Genetic diversity, Inter-simple sequence repeat (ISSR), Core collection,

摘 要 ：为了评价、管理和利用抗癌植物冬凌草(Rabdosia rubescens)的种质遗传资源，采用ISSR分子标记对其7个自然居群进行了遗传多样性分析。结果表明：冬凌草在物种水平具有中等的遗传多样性(Ppl：60.45%，H：0.2714，I：0.3365，HB：0.3108)，在居群水平具有较低的遗传多样性(Ppl：17.05%， H：0.0692， I：0.0989，HB：0.0763)，居群间存在较高的遗传分化(GST：0.6515，θB：0.6388，GST-B：0.6095)。基于遗传距离的UPGMA聚类中，位于华西的四川(SC)居群和中南的保靖(BJ)和石门(SM)居群组成核心分支，再分别与向南和向北的居群聚合。因此，ISSR分子标记适合于冬凌草遗传多样性研究，对其核心种质库的构建具有指导作用。

Abstract:Rabdosia rubescens (Henmsl.) Hara is an anticancer plant distributed in the hills and mountains of mainland China. In order to evoluate, manage and develop the germplasm genetic resources of this species, 7 natural populations were sampled and analyzed using Inter-simple sequence repeats (ISSR) markers. There was a moderate level of diversity at species level (Ppl: 60.45%; H: 0.2714; I: 0.3365; HB: 0.3108), and a low level of diversity at population level (Ppl: 17.05%; H: 0.0692; I: 0.0989; HB: 0.0763), and a high level of genetic differentiation (GST: 0.6515; θB: 0.6388; GST-B: 0.6095). The Sichuan (SC), Baojing (BJ) and Shimen (SM) populations composed core clade, and then gathered with the southern and northern populations, respectively, based on the UPGMA analysis. Therefore, ISSR markers were suitable for genetic diversity analysis and instructive for core collection construction of R.. rubescens.

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