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作 者 ：刘海燕,冯冬茹,刘兵,何炎明,王金发,王宏斌

期 刊 ：热带亚热带植物学报 2009年 17卷 3期 页码：218~222

关键词：MpASR蛋白；亚细胞定位；洋葱；内表皮细胞；绿色荧光蛋白；农杆菌；

Keywords:MpASR, Subcellular localization, Onion, Epidermic cell, GFP, Agrobacterium,

摘 要 ：通过比较不同侵染液浓度、侵染时间、取材部位、预处理等条件下的转化效果，优化了农杆菌介导的洋葱鳞茎内表皮细胞转化系统，并且成功检测到大蕉ASR蛋白(MpASR)与GFP的融合蛋白在洋葱表皮细胞中的分布。

Abstract:The effects of several factors on onion (Allium cepa L.) epidermal cells transformation were studied, including concentration of infection solution, infection time, parts of material and pre-treatment. The transformation system for onion epidermal cells mediated by Agrobacterium was established. The location of MpASR from Musa sapientum fused with GFP in onion epidermal cells was detected successfully.

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