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作 者 ：李媛媛；项凤影；高悦；祖元刚*；路祺；张莹

期 刊 ：植物研究 2014年 34卷 6期 页码：861-865

关键词：杜仲；水浴；总固形物；活性成分；

Keywords:Eucommia, water-bath, total solid, active component,

摘 要 ：

以杜仲鲜皮作为原料，水为提取溶剂，采用单因素实验方法探讨料液比（1∶5~1∶60 g·mL^-1）、提取时间（10~120 min）和提取温度（30~90℃）对杜仲皮总固形物得率及活性成分提取率的影响，并确定单因素实验最佳提取工艺为：料液比1∶20，提取时间60 min，提取温度60℃。此条件下，总固形物得率为11.61%，其中活性成分绿原酸得率为0.073%，桃叶珊瑚苷得率为0.704%，京尼平苷得率为0.122%，松脂醇二葡萄糖苷得率为0.108%。水提取杜仲皮成分，方法简单，无环境污染和溶剂回收问题，本文为杜仲鲜皮活性成分提取和开发利用提供的实验依据。

Abstract:We investigated the total solid and the active component yields from fresh Eucommia bark by singlefactor experiments of aqueous extraction under the liquid ratio of 1∶20 to 1∶60 g·mL^-1, extraction time from 10-120 min, and extraction temperature from 30℃ to 90℃. Under the optimum extraction processes of singlefactor experiment, the total solid yield was 11.61%, and the extraction yields of chlorogenic acid, aucubin, geniposide and pinoresinol diglucoside were 0.073%, 0.704%, 0.122% and 0.108%, respectively. Aqueous extraction has advantages of simple process without environmental pollution and solvent recovery.

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