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作 者 ：杨桂燕1,2；郭宇聪1；赵玉琳1；张凤娇1；高彩球1*

期 刊 ：植物研究 2015年 35卷 5期 页码：692-695

关键词：刚毛柽柳；表达分析；c亚基；V-ATPase；ThVHAc1；

Keywords:Tamarixhispida, expressionanalysis, csubunit, V-ATPase, ThVHAc1,

摘 要 ：V-ATPase是多亚基复合蛋白，其c亚基负责V-ATPase的组装及质子通道的形成。本研究拟分析盐胁迫下过表达ThVHAc1基因拟南芥V-ATPase各亚基的表达，探讨过表达外源c亚基对拟南芥V-ATPase全酶响应盐胁迫表达模式的影响。实时荧光定量PCR结果显示，盐胁迫下，过表达外源ThVHAc1拟南芥V-ATPase 28个亚基的表达发生了明显改变，且拟南芥5个c亚基的表达均不同程度的被抑制。表明外源ThVHAc1基因能影响拟南芥V-ATPase各亚基的表达以调节V-ATPase全酶的活性，但各亚基的表达模式与V-ATPase活性非简单对应关系，各亚基互相协调决定V-ATPase活性。

Abstract:V-ATPase is a muti-subunits protein complex, the c subunit is mainly responsible for V-ATPase assembly and proton channel formation. We measured the expression profiles of V-ATPase subunits in exogenous overexpression ThVHAc1 Arabidopsis under salt by using real time RT-PCR. Twenty-eight V-ATPase subunits were all effected by exogenous ThVHAc1, and five Arabidopsis c subunits were down-regulated expression, indicating that ThVHAc1 affected the V-ATPase activity by altered the expression of V-ATPase all subunits. Between the expression of V-ATPase subunit and V-ATPase activity showed non-monolithic relationship, they were functioned together coordinately on V-ATPase to response to slat stress.

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