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作 者 ：刘梦然;边秀艳;麻文俊;邵龙婷;姚立新;孟丙南;刘桂丰;赵曦阳*

期 刊 ：植物研究 2014年 34卷 4期 页码：485-491

关键词：TaLEA；转基因；Logistic；模型；变异；

Keywords:TaLEA, transgene, Logistic, model, variation,

摘 要 ：以10个转TaLEA小黑杨无性系和1个对照无性系为材料，对无性系苗期苗高和地径生长过程进行测定并构建Logistic模型，探讨不同无性系苗期生长过程的变异情况。结果表明：转基因小黑杨无性系苗高和地径生长过程高度符合“S”型曲线，所有Logistic模型拟合系数超过0.9，表明拟合效果显著。方差分析结果表明不同无性系苗高和地径间差异均达到极显著水平（P＜0.01）。参试无性系苗高平均速生期持续期为48天，速生期内平均生长量为1.85 cm，地径平均速生期持续时间为70天，速生期内平均生长量为0.09 mm。苗高、地径均与速生期内生长量、速生期内平均日生长量间呈极显著正相关。转基因无性系XL-1和XL-9苗期生长较快，苗高（地径）分别比对照高16.37%（32.82%）和10.92%（22.68%），表明转LEA基因对无性系生长具有一定的影响。此研究为小黑杨苗期管理提供理论依据。

Abstract:A total of 10 transgene and one control Populus simonii×P.nigra clones were used to investigate and analyze the variation of growth curve of tree height and basic diameter. The result showed that tree height and basic diameter growth process curve accord with “S” shape. The coefficient of Logistic model was higher than 0.9 which indicated that the function of the model was effective. There were remarkable differences within different variation sources between the tree height and basic diameter of different clones (P＜0.01). The average rapid growth last for 44 d for tree height and 70 d for basic diameter, respectively. The average increments during rapid growth period were 1.85 cm and 0.99 mm for tree height and basic diameter, respectively. There were remarkably positive correlation coefficients between tree height, basic diameter, average increment during rapid growth period and average increment each day during rapid growth period. XL-1 and XL-9 grew fast, tree heights (basic diameters) were 16.73%(32.82%) and 10.92%(22.68%) higher than control, respectively, which indicated transgene remarkably affect tree growth and this research can provide fundamental basis for future research.

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