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作 者 ：李芳1；肖小君1*；林忠全2；杜明林1；胡章薇1；黄作喜1

期 刊 ：植物研究 2015年 35卷 6期 页码：825-831

关键词：白芨；再生体系；优化；

Keywords:Bletilla striata, regeneration system, optimization,

摘 要 ：以野生红花白芨成熟蒴果为外植体，建立了白芨植株的再生体系。结果表明：白芨种子萌发最佳培养基为1/2 MS+6-BA 1.0 mg·L^-1+NAA 0.1 mg·L^-1+AC 0.5 g·L^-1；原球茎增殖最适培养基为MS+KT 1.0 mg·L^-1+NAA 0.1 mg·L^-1+AC 0.5 g·L^-1；原球茎分化培养基为MS+6-BA 0.5 mg·L^-1+KT 1.0 mg·L^-1+NAA 0.1 mg·L^-1+AC 0.5 g·L^-1；不定芽增殖最佳培养基为MS+6-BA 0.5 mg·L^-1+KT 1.0 mg·L^-1+NAA 0.1 mg·L^-1+AC 0.5 g·L^-1 +土豆汁150 g·L^-1，30 d后的芽增殖率达343%；最适生根培养基为1/2MS+IBA 0.5 mg·L^-1+AC 0.5 g·L^-1+土豆汁100 g·L^-1，生根率达100%；试管苗移栽的最佳方式是以水苔+树皮+椰糠+锯木粉(2∶2∶1∶1，V/V/V/V)为混合基质进行多株移栽，成活率可达99%。

Abstract:We used the ripe capsules from wild Bletilla striata as explants to establish regeneration system. The optimal medium for seed germination was 1/2MS+6-BA 1.0 mg·L^-1+NAA 0.1 mg·L^-1+AC 0.5 g·L^-1. The most suitable medium for PLB multiplication was MS+KT 1.0 mg·L^-1+NAA 0.1 mg·L^-1+AC 0.5 g·L^-1, while MS+6-BA 0.5 mg·L^-1+KT 1.0 mg·L^-1+NAA 0.1 mg·L^-1+AC 0.5 g·L^-1 was appropriate for PLB differentiation. The optimal medium for adventitious buds multiplication was MS+6-BA 0.5 mg·L^-1+KT 1.0 mg·L^-1+NAA 0.1 mg·L^-1+AC 0.5 g·L^-1+potato juice 150 g·L^-1, and the proliferation rate was up to 343% after 30 days. The most suitable rooting medium was 1/2MS+IBA 0.5 mg·L^-1+AC 0.5 g·L^-1+potato juice 100 g·L^-1 with the rooting rate of 100%. The best way of tube seedlings transplanting was under the volume ratio of sphagna: batk: Coconut chaff: wood flour of 2∶2∶1∶1 with 99% of survival rate.

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