---

作 者 ：郭君瑜;张琴;韩榕*

期 刊 ：植物研究 2009年 29卷 2期 页码：204-208

关键词：He-Ne激光；UV-B辐射；小麦；叶绿体；光合系统；

Keywords:He-Ne laser, UV-B, Wheat, Chloroplast, The system of photosynthesis,

摘 要 ：对“晋麦8号”小麦幼苗分别采用5 mW·mm^-2 He-Ne激光辐照、10.08 kJ·m^-2·d^-1增强UV-B辐射及二者组合进行处理,研究各处理组小麦幼苗叶绿体膜透性、叶绿体蛋白质含量以及叶绿体偶联因子CF-1的ATP酶活性、希尔反应的活性变化。结果表明：UV-B辐射后小麦幼苗叶绿体膜透性增加，叶绿体蛋白质含量有一定的下降,而ATP酶活性、希尔反应的活性均受到抑制。经过He-Ne激光辐照可使叶绿体膜透性降低、叶绿体蛋白质含量有一定的升高,同时ATP酶活性、希尔反应的活性也受到部分激活。这些变化说明增强UV-B辐射引起小麦幼苗叶绿体损伤,而一定剂量的He-Ne激光辐照可部分修复增强UV-B对小麦幼苗光合系统的损伤。

Abstract:The seedling of wheat (jinmai8) were exposed to He-Ne laser (5 mW·mm^-2), enhanced UV-B radiation (10.08 kJ·m^-2·d^-1) and the combined treatment of He-Ne laser irradiation and enhanced UV-B radiation. The measurement permeability of chloroplast membrane，chloroplast protein content，Hill reaction activity and ATPase activity of wheat seedlings. Result enunciation: the chloroplast membrane permeability has increased after UV-B radiated wheat seedlings，chloroplast protein content went down, Hill reaction activity and ATPase activity were restrained. The He-Ne laser can make chloroplast membrane permeability fall，chloroplast protein content go up certainly. Hill reaction activity and ATPase activity were also partially activated.These variety explain enhanced UV-B radiation cause damage chloroplast of wheat seedling, but certain the He-Ne laser partially repair UV-B damage the system of photosynthesis on wheat seedling.

---