摘 要 ：建立了反相高效液相法测定长春花中吲哚类生物碱文多灵、长春质碱和阿玛碱含量的方法，色谱柱为HiQ sil C18色谱柱（250 mm×4.6 mm，5 μm）；流动相为1%二乙胺水溶液(磷酸调pH=7.2)—甲醇—乙腈/2:1:1(V/V)；流速为1 mL·min-1；检测波长为215 nm；柱温为40℃。并采用此方法对长春花根、茎、叶、花和种子以及不同产地的长春花中的这3种生物碱进行了检测，结果表明文多灵和长春质碱主要存在于在根、茎、叶、花中，阿玛碱主要存在于种子中；并且随着地理位置的北移，长春花中的文多灵和长春质碱的含量逐渐降低，温室中人为控制长春花中的栽培条件能提高二者的含量。
Abstract:A RP-HPLC quantification method was established to determine the contents of three indole alkaloids-vindoline, catharanthine and ajmalicine in Catharanthus roseus. HiQ sil C18 column(250 mm×4.6 mm，5 μm) was used. The mobile phase was 1% diethylamine(pH=7.2)-methanol-acetonitrile/2:1:1(V/V). The detection wavelength was 215 nm. The flow rate was 1 mL·min-1. The column temperature was 40℃. Different parts of C. roseus were determined and the results showed that vindoline and catharanthine mainly existed in roots, stems, leaves and flowers, while ajmalicine mainly existed in seeds. C. roseus from different habitats were also determined by this method and the results showed that the contents of vindoline and catharanthine in south habitats samples were higher than those in north habitats. For the cultured C. roseus in greenhouse, both vindoline and catharanthine contents were enhanced greatly.