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作 者 ：于淼;赵权;王军;*

期 刊 ：植物研究 2012年 32卷 2期 页码：183-190

关键词：葡萄；花色苷；乙烯利；基因表达；

Keywords:grapevine, anthocyanin, ethephon, gene expression,

摘 要 ：利用荧光定量PCR技术分析‘京优’葡萄果实成熟过程中，花色苷生物合成途径相关酶基因mRNA转录水平的变化以及乙烯利处理对果皮中花色苷含量和关键酶基因转录水平的影响。结果显示，葡萄果实发育进入着色期，花色苷合成过程中主要相关基因(CHSs、CHIs、F3Hs、F3′H、F3′5′H、DFR、LDOX、UFGT、OMT和GST)和转录因子(MybA1和MybA1-2)转录水平都显著提高，其中UFGT、GST、MybA1和CHSs、CHIs、F3Hs基因家族中的CHS3、CHI2、F3H2随着花色苷合成而大量转录；乙烯利处理能够增强花色苷合成相关基因的转录，使其转录时期前移和转录水平提高，其中对GST、UFGT和MybA1转录的促进作用最明显。相关性分析表明，花色苷合成与一些花色苷合成相关基因(CHS3、CHI2、F3H2、F3′5′H、UFGT、GST)和转录因子(MybA1)的转录水平呈显著或极显著正相关；与CHS1、CHS2、CHI1、F3H1、DFR、F3′H、LDOX和OMT转录水平的相关性均不显著。本研究结果为进一步阐明花色苷生物合成机理和花色苷类色素的生产应用提供一定的理论依据。

Abstract:The present study investigated the effects of different concentrations of ethephon on the changes of anthocyanin contents and the transcription of anthocyanin biosynthesis enzyme genes during the fruit coloring period in Jingyou grapevine by means of real-time PCR. The results demonstrated that the anthocyanin biosynthesis pathway genes, including CHSs, CHIs, F3Hs, F3′H, F3′5′H, DFR, LDOX, UFGT, OMT, GST and transcriptional factors (MybA1, MybA1-2) were up-regulated expressed at veraison. The transcription of CHS3, CHI2 and F3H2 in gene families of CHSs, CHIs and F3Hs is closely related to the anthocyanin synthesis. Besides, UFGT, GST and MybA1 were transcribed in great quantity with anthocyanin synthesizing. The transcription of the anthocyanin biosynthesis pathway genes was enhanced by ethephon treatments, which accelerated transcribing earlier and enhanced the quantity of transcription, especially GST, UFGT and MybA1. Correlation analysis of the principal related genes and anthocyanins demonstrated that the genetic transcriptional level of some anthocyanin biosynthesis genes (CHS3, CHI2, F3H2, F3′5′H, UFGT and GST) and transcription factor (MybA1) were positively correlated to the content of anthocyanin. The genetic transcriptional level of CHS1, CHS2, CHI1, F3H1, DFR, F3′H, LDOX and OMT was not correlated to the content of anthocyanins. The results of this experiment will provide a theoretical basis for further clarifying the mechanism of anthocyanin biosynthesis and application.

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