---

作 者 ：李刚;刘晓颖;李学平;王振英*

期 刊 ：植物研究 2010年 30卷 4期 页码：441-447

关键词：TCTP基因；白粉菌；表达半定量分析；抗白粉病栽培小麦；

Keywords:translationally controlled tumor protein gene, Erysiphe graminis, semiquantitative PCR analysis, powdery mildew resistant cultivar wheat Brock,

摘 要 ：翻译控制肿瘤蛋白(translationally controlled tumor protein，TCTP)最初在鼠肿瘤细胞中被发现，研究表明TCTP广泛存在于动植物细胞中，并具有多种生物学功能。本研究用RTPCR和RACE技术在抗白粉病栽培小麦Brock中克隆了一个TCTP基因，该基因全长766 bp，推测编码一个168个氨基酸的多肽。ScanProsite分析表明，该多肽链具有2个TCTP特征结构区（TCTP1和TCTP2）和7个可能的功能位点。表达半定量分析发现，该基因受华北地区流行的优势小种15号白粉菌诱导，且随着诱导时间的增加其表达量增加。本研究将可能在小麦白粉病抗性研究领域开辟新的研究思路。

Abstract:The translationally controlled tumor protein (TCTP) was initially designated as a find in mouse tumor cells. It is abundant in plant and animal cells and has many different functions. In this study, a TCTP gene was isolated as an Erysiphe graminis inducible cDNA cloned from the leaves of cultivar wheat Brock infected by E.graminis with RT-PCR and RACE technique. It encoded a polypeptide of 168 amino acids. After running a ScanProsite research, two specific TCTP signatures, named TCTP1 and TCTP2 and 7 conservation function sites were found. The studies on the expression of WbTCTP after infected by powdery mildew of race No.15 were carried out using sei-quantitative PCR technique. The expression level of WbTCTP gradually increased during the E.graminis infection. The results would provide a new way for the area of studying wheat powdery mildew resistance.

---