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作 者 ：魏占才;周 鑫

期 刊 ：植物研究 2006年 26卷 4期 页码：427-429

关键词：丽格海棠；组培苗；离体生根；

Keywords:Begonnia×Rieger, regenerated shoots, in vitro rooting,

摘 要 ：为了建立有效的丽格海棠组培苗离体生根系统，降低培育丽格海棠组培苗离体生根的成本，对基本培养基的不同离子强度、白糖浓度以及培育琼脂浓度对丽格海棠的组培苗离体生根进行了研究。研究发现，培养基的基本离子强度和白糖浓度对丽格海棠组培苗的离体生根有一定的影响，从降低成本的角度考虑，1/4MS培养基为最佳的培养基，15 g·L^-1的白糖为最佳的白糖浓度。所采用的琼脂浓度对丽格海棠组培苗的离体生根没产生明显的影响，从降低成本的角度选择3 g·L^-1的琼脂为最佳的琼脂浓度。

Abstract:For establishing an efficient method in vitro rooting protocol for regenerated shoos of Begonnia×Rieger and decreasing the cost of rooting, the effects of basal medium strength, white sugar concentration and agar concentration were investigated in this study.The results showed that the strength of basal medium and white sugar concentration produced more effects on rooting. Considering rooting frequency and rooting quality, 1/4MS basal medium and 15 g·L^-1 white sugar were the best choice for rooting of Begonnia×Rieger. In all treatment of different agar concentration, there were no obvious difference in rooting frequency and rooting quality, for decreasing the cost, we selected 3 g·L^-1 agar as the best concentration for rooting.

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