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作 者 ：杨玲;沈海龙*;刘春苹;翟晓杰

期 刊 ：植物研究 2010年 30卷 2期 页码：174-179

关键词：花楸；合子胚；胚性愈伤组织；体细胞胚发生；

Keywords:Sorbus pohuashanensis, zygotic embryo, embryogenic callus, somatic embryogenesis,

摘 要 ：分别以完整成熟胚、切去一个子叶的成熟胚和切下的子叶为外植体，以MS为基本诱导培养基、1/2MS为基本分化培养基，进行了花楸体细胞胚胎发生研究。结果表明：以完整合子胚作为外植体的体胚诱导率最高，为100%，最佳植物生长调节剂组合为5 mg·L-1 NAA+2 mg·L^-1 6-BA；NAA和6-BA浓度及二者的交互作用对愈伤组织和体胚诱导率的影响极显著；光照配合延长继代间隔时间有利于体胚发生。实体观察结果表明，花楸体胚发生方式有直接发生和间接发生两种；体胚发育经历了球形期、心形期、鱼雷形期和子叶期。组织学观察结果表明，体胚具有两极性，子叶期体胚结构完整。

Abstract:Somatic embryogenesis of Sorbus pohuashanensis Hedl was studied by taking intact mature zygotic embryo, zygotic embryo with 1 cotyledon and the cut cotyledon as explants. The basal induction and differentiation media were MS and MS1/2, respectively. The results showed that somatic embryo induction rate was the highest on intact mature zygotic embryo explant, which was 100%，and the best combination of plant growth regulators was 5 mg·L^-1 NAA plus 2 mg·L^-1 6-BA for induction of somatic embryos on MS medium. The concentrations of NAA and 6-BA and their interaction have significant effects on induction of callus and somatic embryos. Light environment with longer subculture interval were favorable to somatic embryogenesis. Stereomicroscope observation showed that there were two regeneration patterns (direct and indirect) and four development stages (globular, heartshape, torpedo-shape and cotyledonary) for somatic embryogenesis of the species. Microscopic observation showed that the somatic embryos had bipolarity, and cotyledonary somatic embryos had fully-developed structure.

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