---

作 者 ：高丽美;李永锋;韩榕*

期 刊 ：植物研究 2010年 30卷 1期 页码：32-36

关键词：He-Ne激光；UV-B辐射；捕光色素；小麦；

Keywords:He-Ne laser, ultraviolet-B radiation, chloroplast pigment, wheat,

摘 要 ：采用5 mW·mm^-2 He-Ne激光辐照、10.08 kJ·m^-2d^-1 UV-B辐射及二者组合对冬小麦幼苗进行处理。通过测定叶绿体捕光色素含量和色素蛋白组成的变化，进一步探讨He-Ne激光对增强UV-B辐射后小麦幼苗类囊体捕光色素损伤的修复效应。循环处理小麦幼苗4 d，利用90%乙醇和80%丙酮分别提取各处理组小麦幼苗叶片中的叶绿素,通过纸层析和分光光度法检测捕光色素含量的变化，并探讨不同处理对叶绿素与蛋白质结合牢固性的影响。利用柱层析法测定色素蛋白的主要成分。研究表明：与对照组相比，增强UV-B辐照后小麦幼苗捕光色素总含量降低了17.76%，叶绿素和蛋白质结合牢固度显著降低,色素蛋白的组成也发生变化，D1和D2蛋白质条带消失；而一定剂量He-Ne激光辐照可使增强UV-B辐射后的叶绿体色素含量增加约10.64%，但仍低于ck组约8.12%,叶绿素和蛋白质结合牢固度也显著高于B组，色素蛋白的组成与对照组相似。因此，低剂量的He-Ne激光辐照对增强UV-B辐射后小麦幼苗类囊体捕光色素的损伤具有促进修复效应。

Abstract:The seedlings of wheat were exposed to He-Ne laser(5 mW·mm^-2) irradiation and enhanced UV-B(10.08 kJ·m^-2d^-1) radiation. Then the content changes of chloroplast pigments and the composition of pigment-proteins were measured in order to test the mitigative effect of He-Ne laser irradiation on injury by enhanced UV-B radiation. After treating wheat seedlings for 4 days circularly, chloroplast pigments were extracted respectively by 90% ethanol and 80% acetone. The contents of chloroplast pigments were detected with spectrophotometer or paper chromatography. The composition of pigmentproteins were detected with column chromatography. It suggested that the content of chloroplast pigments decreased 17.76% approximately in wheat seedlings by enhanced UV-B radiation. The binding degree of Chl-Pro is weakened. The composition of pigment-proteins also differed from the control group. Straps of D1 and D2 proteins lost. However, the content of chloroplast pigments increased about 10.64% exposed to He-Ne laser irradiation, but decreased 8.12% comparing with the control. The binding degree of Chl-Pro is higher than the B group. At the same time, the composition of pigmentproteins was similar with the control group. So the lower dose of He-Ne laser irradiation can partly repair the radiation injury on chloroplast pigments and pigment-proteins in wheat seedlings after enhanced UV-B radiation.

---