Abstract:The microcharacteristics of leaf epidermis of Tilia amurensis and T. mandshurica were observed by scanning electron microscope, and it’s microcharacteristics was studied comparatively. The results indicated that the microcharacteristics of up-epidermis of leaf of T. amurensis were the same with T. mandshurica, the waxiness emblazonry, the morphological character and the quantity of stelllate hair in elemental area of stomatal apparatus in down-epidermis of leaf showed great differences. These differences of microcharacteristics could be an anatomy proof to diefferentiate T. amurensis and T. mandshurica.