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作 者 ：栗孟飞;李唯;杜微;李金娟

期 刊 ：植物研究 2010年 30卷 2期 页码：215-220

关键词：桃儿七种子；休眠机理；对苯二酚；生物活性鉴定；植物生长激素；

Keywords:Podophyllum hexandrum Royle seed, dormancy mechanism, hydroquinone, bioactivity identification, auxin,

摘 要 ：为了进一步了解濒危植物桃儿七种子休眠机理，采用气质联用仪（GC-MS）对桃儿七种子的乙醚浸提物进行分离与鉴定。结果表明，经GC-MS分离与鉴定出脂肪类、烷类，酸类等19种化合物中，多数化合物已被前人研究证明具有生物抑制活性，这也正是桃儿七种子萌发不良、休眠产生的主要原因及机理。对苯二酚是首次从桃儿七种子中分离出的有机化合物，利用对苯二酚对小麦进行生物活性的鉴定显示，0.1^-1～1.0 mg·L^-1的对苯二酚可显著促进小麦的生长。通过在桃儿七种子分离胚培养基中添加1.0 mg·L^-1的对苯二酚，可显著促进无菌苗的生长和发育，缩短生长和发育周期，并且植株生长健壮，根系发达。由此可说明，对苯二酚在促进植物的生长和发育方面具有类似于植物生长激素的作用。

Abstract:Ethyl ether extract from seed of Podophyllum hexandrum Royle was separated and identified by GC-MS, nineteen compounds existed including fatty acid, alkenes and others, most of which had been proved to possess inhibition activities that might be the primary reason of unhealthy germination of P.hexandrum Royle seed. Hydroquinone was firstly identified in this experiment and its bioactivity had never been reported in the reference before. The further study of its bioactivity was conducted on wheat and in vitro culture of P.hexandrum Royle seed. The results showed that hydroquinone(1×10^-1～1.0 mg·L^-1) played a positive role and shortened the cycle in growth and development, which indicates that hydroquinone has similarity to auxin on plant growth and development.

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