Abstract:Using ISSR method, the genetic diversity and genetic structure of 100 individuals in four natural populations of Chenopodium album L. distributed in Dalian surrounding were studied in this paper. Through the amplification of ISSR with 13 primers, 157 loci were detected and the percentage of polymorphic loci was 67.52. Shannon’s information index was 0.332 9 at the species level, genetic variance was 40.03% among populations according to GST value. The genetic distances analysis showed that DT population and MLN population have the highest population genetic identity, genetic distances and geographic distances have no correlation.