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作 者 ：孔冬梅;万婷

期 刊 ：植物研究 2010年 30卷 6期 页码：668-673

关键词：油松；微繁；不定芽；生根；炼苗；

Keywords:Chinese pine, micropropagation, adventitious bud, rooting, acclimatization,

摘 要 ：以油松（Pinus tabulaeformis Carr.）成熟合子胚为外植体进行了直接器官发生研究。在添加1~5 mg·L^-1 BA，或再配合添加1～10 mg·L^-1 2,4-D的DCR和MS培养基上均能诱导发生不定芽。在只添加1 mg·L^-1 BA的DCR培养基上不定芽诱导率最高，为90%。及时将不定芽继代于不含生长调节物质的DCR培养基上有利于芽的伸长。添加1 mg·L^-1 IBA的1/2DCR培养基最适于不定根的诱导，在该培养基上不定根诱导率达到47.1%。再生植株在蛭石基质上炼苗后转到温室培养，成活率高达86.4%，且生长正常。

Abstract:Mature zygotic embryos of Chinese pine were cultured as initial explants to investigate the process of direct organogenesis. Adventitious buds were initiated on DCR and MS medium supplemented with 1~5 mg·L^-1 benzyladenine (BA) with or without 1~10 mg·L^-1 2,4-dichlorophenoxyacetic acid (2,4-D). The highest induction frequency of adventitious buds (90%) was obtained on DCR medium containing 1 mg·L^-1 BA. Subsequent transfer of buds to plant growth regulator (PGR)-free medium in time was advantageous for elongation of adventitious buds. 1/2DCR medium with 1 mg·L^-1 indole-3-butyric acid (IBA) was suitable for adventitious root initiation, on which average rooting frequency was 47.1%. Plantlets were successfully acclimatized in vermiculite and transferred to greenhouse, where they had a survival rate of 86.4% and no morphological abnormalities.

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