摘 要 ：利用RT-PCR和RACE技术从斑茅（Erianthus arundinaceus）中分离出编码鸟氨酸-δ-氨基转氨酶基因的全长cDNA序列，序列全长1 680 bp，编码454个氨基酸。通过对哺乳动物、高等植物、微生物的δ-OAT基因编码的氨基酸序列进行同源比对，发现斑茅δ-OAT基因同其近缘属植物甘蔗的同源性最高（87%），同其他高等植物的同源性次之（约为70%），而同动物的同源性最低（约为60%）。在斑茅δ-OAT基因编码的氨基酸序列的5′端未发现线粒体定位序列，同甘蔗δ-OAT基因一样。斑茅δ-OAT基因具有完整的鸟氨酸转氨酶功能区rocD。利用定量RCR（real-time PCR）对30%PEG胁迫下的斑茅δ-OAT基因表达量进行研究，结果表明δ-OAT基因在胁迫12 h表达量达到最高，约为对照的4.1倍；胁迫2 h δ-OAT基因表达量反而有所降低。
Abstract:The complete cDNA sequence of orn-δ-aminotransferase (OAT) gene was obtained from Erianthus arundinaceus and was cloned by reverse-transcript-polymerase-chain-reaction (RT-PCR) and rapid amplification of cDNA end (RACE) technologies. The acquired gene was 1 680 bp in full length, encoding 454 amino acid residues. The amino acid sequence blast results showed that compared to that from mammal, higher plant and microorganism, δ-OAT gene from E.arundinaceus shared the highest homology (87%) with relative genera plant, Saccharum officinarum, 70% homology with other higher plants and 60% homology with animal. No N-terminal mitochondrial transit peptide (MTP) was found in the amino acid sequence encoded by δ-OAT gene from E.arundinaceus, which was the same as that from S.officinarum. Complete domain of OAT, rocD, was included in δ-OAT gene from E.arundinaceus. Expression level of δ-OAT gene from E.arundinaceus treated with 30% polyethylene glycol (PEG) was studied using real-time PCR technology, which showed that after treated 12 hours with PEG, the expression level reached the highest, 4.1 times as that of the comparison, but got lower after stressed 2 hours.