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作 者 ：宰学明;朱士农;钦佩;袁亚光;吴向华;周文宗

期 刊 ：植物研究 2011年 31卷 6期 页码：735-738

关键词：水芹；水浸提液；斜生栅藻；生长；叶绿素；超微结构；

Keywords:Oenanthe javanica, extracts, Scenedesmus obliquus, growth, chlorophyll, ultrastructure,

摘 要 ：采用栅藻在不同浓度的水芹水浸提液中纯培养的方法，研究了水芹水浸提液对斜生栅藻细胞数、叶绿素含量及藻细胞超微结构的影响。结果显示，10 g·L^-1水芹水浸提液对斜生栅藻的生长和叶绿素含量的增加具有明显的促进效应；20 g·L^-1水芹水浸提液对藻细胞数和叶绿素含量的增加持续到第3 d，3 d后出现显著的抑制效应；高浓度（30~50 g·L^-1）水芹水浸提液对藻细胞数和叶绿素含量的明显抑制在第2 d始现，随时间延长而加剧，并具有浓度效应；40 g·L^-1水芹水浸提液处理后，斜生栅藻细胞壁断裂甚至消失，细胞中叶绿体片层肿胀甚至解体，核膜破裂，核质外渗。水芹水浸提液对斜生栅藻具有化感效应，低浓度促进生长，高浓度抑制。

Abstract:Using pure culturing Scenedesmus obliquus with different concentrations of Oenanthe javanica extracts, the effects of O.javanica on the growth, chlorophyll content and structure of S.obliquus were studied. The results showed that 10 g·L^-1 O.javanica extracts significantly promoted the growth of S.obliquus, and 20 g·L^-1 O.javanica extracts increased the algae chroma and chlorophyll content of S.obliquus till the third day, and then caused obvious inhibition. 30-50 g·L^-1 O.javanica extracts inhibited the algae chroma and chlorophyll content observably on the second day, and intensified with time extension and concentration increase. When the growth of S.obliquus was affected by 20 g·L^-1 O.javanica extracts, the cell wall broke and even disappeared, cells of chloroplast swelled and even disintegrated, the nuclear broke, and the nuclear mass exosmosed. The results indicated that O.javanica extracts had allelopathy on S.obliquus, and lower concentrations promoted the growth, while higher concentrations inhibited.

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