Abstract:Using Asparagua officinalis stem shot as explant, the paper discussed the factors that affected the rooting in vitro. The concentration of sucrose, abiosalt and plant growth regulator were studied. The result indicated that 1/2MS and 20g·L-1 sucrose promoted rooting;Rooting rate arrived at 100% when it was pretreated in the twelfth with 1/4MS and 20 g·L-1 sucrose. Its quality of root excelled the root in the medium with hormone;pretreatment of hormone couldn’t enhance rooting rate.Therefore,low salt or low sucrose pretreatment was a kind of good way for advancing rate of rooting in Asparagua officinalis.