Abstract:In order to evaluate the resistance of Galium aparine to tribenuron-methyl, 14 potential resistant biotypes were collected from winter wheat fields of 14 counties or cities in Shandong, Shanxi, Henan, Hebei, Jiangsu, Shaanxi, and Anhui Provinces, and 14 susceptible biotypes were correspondingly collected from the nearby non-farming lands never applied herbicides. Greenhouse pot tests and the quick-test in Petri dish were conducted to detect the resistance level. The results of greenhouse pot tests showed that except for the potential resistant biotypes from the collecting sites of Shijiazhuang, Hebei Province, Taiyuan, Shanxi Province, Zhouzhi, Shaanxi Province, and Tai’an, Shandong Province, all the other potential resistant biotypes evolved resistance to tribenuron-methyl, with a resistance factor ranging from 1.6 to 4.3, with the highest 4.3 in the collecting site of Xuchang, Henan Province and the lowest 1.6 in the collecting sites of Taihe, Anhui Province and Huaxian, Shaanxi Province. The resistance in the collecting site of Xuchang, Henan Province is relatively higher than those in the collecting sites of Taihe, Anhui Province and Huaxian, Shaanxi Province, with a resistance factor 2.2, 1.9, 1.7 respectively. The results of the quick-test in Petri dish are in accordance with those of greenhouse pot tests on the whole.