Abstract:Soybean root rot caused by Phytophthora sojae is a destructive disease. In order to understand molecular mechanisms of soybean resistance to Phytophthora sojae, the gene expression in hypocotyls and stems of variety ‘Suinong 10‘ were investigated by using mRNA differential display reverse transcription PCR at 0, 0.5, 1, 2 and 4h after inoculation with zoospores of P.sojae race 1. The results showed that at least eight differential fragments at the transcriptional level were related to resistance genes. These fragments represented cinnamic acid 4-hydroxylase gene, Atp β gene coding ATP synthase β subunit and ubiquitin-conjugating enzyme gene which upregulated at 0.5h post inoculation, UDP-N-acetyl-alpha-D-galactosamine gene which upregulated at 1h post inoculation and blue copper protein gene which upregulated at 2h post inoculation, TGA-type basic leucine zipper protein gene, cyclophilin gene and 14-3-3 protein gene which upregulated at 4h post inoculation. The results suggested among these eight genes, one related to signal transduction, four related to resistance and defense, two related to transcription and regulation, and one related to energy metabolism. The results showed that aforesaid genes played important roles during zoospore germination, penetration and mycelium growth of P.sojae in soybean.