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作 者 ：叶建仁 黄素红 李传道 程淑婉

期 刊 ：林业科学 1994年 30卷 5期 页码：430-436

关键词：松针褐斑病；湿地松；6-磷酸葡萄糖脱氢酶；苯丙氨酸解氨酶；抗病机制；

Keywords:Brown spot needle blight, Pinus elliottii, Glucose-6-phosphate dehydrogenase, Phenylalanine ammonia lyase, Resistance machanism,

摘 要 ：松针褐斑病(Lecanosticta acicola)是我国南方重要病害。在湿地松(Pinus elliottii)抗褐斑病选育研究的基础上对湿地松针叶中的6-磷酸葡萄糖脱氢酶和苯丙氨酸解氨酶与抗病性的关系进行了研究分析。结果表明二年生健康针叶的6-磷酸葡萄糖脱氢酶的活性水平与针叶的抗病性有密切关系。在测定的13个无性系和单株中,4个易感株的酶活性较低,9个抗病无性系中除个别酶活性与易感株相近外,其余无性系的酶活性和比活性都明显比易感株高。苯丙氨酸解氨酶的株间差异相对较小,但在总体水平上,抗病无性系的酶活性和比活性也比易感株略高。用松针褐斑病菌毒素液处理后,无论是抗病针叶还是易感针叶,6-磷酸葡萄糖脱氢酶的活性均呈上升趋势,升幅为0.5—1倍,但苯丙氨酸解氨酶的活性在用毒素处理后无明显变化。

Abstract:Brown spot needle disease is one of important diseases on Pinus elliottii and P.taeda in south China.On the basis of resistance selection,the glucose-6-phosphate dehydrogenase and phenylalanine ammonia lyase activities in needles of 9 resistant clones and 4 susceptible individuals of slash pine were studied and analyzed.The results showed that there were close relations between the activity levels of glucose-6-phosphate dehydrogenase and disease resistance in two-year-old needles of slash pine.The enzyme activity levels of 9 resistant clones were mostly higher than those of 4 susceptible individuals.Phenylalanine ammonia lyase activities of the resistant clone needles,in general,were also a bit higher than those of susceptible individuals.After resistant and susceptible needles were treated by primary toxin of brown spot fungus,their glucose-6-phosphate dehydrogenase activities all increased,and the increment extents were all about 0.5-1 times,but their phenylalanine ammonia lyase activities didn‘t evidently change.

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