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期 刊 ：植物保护学报 2010年

关键词：红棕象甲；金龟子绿僵菌；鉴定；rDNA-ITS序列；

Keywords:Rhynchophorus ferrugineus, Metarhizium anisopliae, identification, rDNA-ITS sequence,

摘 要 ：为明确一株红棕象甲寄生真菌的归属,采用形态学及分子生物学方法,从产孢表型、分生孢子特征和ITS序列测定等方面对其进行了分析鉴定。结果表明:该菌分生孢子梗粗短,顶端具2～5个瓶状小梗,分生孢子呈长椭圆形或圆柱形,大小为4.8～9.3μm×2.2～3.1μm;ITS通用引物扩增该菌得到了预期的558bp条带,其与金龟子绿僵菌小孢变种的支持率高达100%。因此,将该红棕象甲寄生真菌菌株鉴定为金龟子绿僵菌小孢变种Metarhizium anisopliae var.anisopliae。

Abstract:A type of entomopathogenic fungus of Rhynchophorus ferrugineus Oliver was identified with morphological and molecular biological methods, including sporulation pattern, conidiospore characteristics and ITS sequence analysis. The results showed that conidiophores of the fungus were tubby and had 2-5 phialides on apex. The conidiospores were long oval or cylindrical, whose size was 4.8-9.3μm×2.2-3.1μm. The expected 558bp band was obtained by ITS universal primer amplification. The results of sequence analysis showed that the pathogenic fungus and Metarhizium anisopliae var. anisopliae were formed a subclade with a bootstrap value of 100%. Based on the above results, the pathogenic fungus of R.ferrugineus was identified as Metarhizium anisopliae var.anisopliae.

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