---

期 刊 ：植物保护学报 2008年

关键词：转Bt基因稻谷；麦蛾；敏感系数；损失率；抗虫性；

Keywords:transgenic Bt rice, Sitotroga cerealella, index of susceptibility, loss rate, resistance,

摘 要 ：麦蛾Sitotroga cerealella Olivier是我国重要的储粮害虫。为系统评价转基因抗虫稻谷对麦蛾的影响,采用室内饲养的生物测定方法,在28±1℃、75％±5％RH条件下,对麦蛾在含水量14％~15％的无虫转Bt基因抗虫稻谷KMD1及其对照品种秀水11中的存活率、子代数、稻谷损失率、敏感系数及各发育指标等进行比较。结果表明:转Bt基因抗虫稻谷对麦蛾具有显著抗性,取食转Bt基因抗虫稻谷的麦蛾羽化率仅为对照的13％,且各发育指标均显著降低,转Bt基因抗虫稻谷的损失率仅为对照的12％,Dobie敏感系数显著低于对照,对麦蛾表现为高抗。

Abstract:Sitotroga cerealella Olivier is an important storage insect pest. Transgenic Bt rice provides a new tool for S.cerealella control. KMD1, a transgenic Bt rice developed by Zhejiang University and expressing Cry1Ab protein, were evaluated for resistance to S.cerealella. Bioassay was carried out in laboratory using artificial rearing with insect-free rice grain which was equilibrated to 14％-15% water content at 75％±5％RH. Under the conditions of 28±1℃ and 75％±5％RH, we evaluated transgenic Bt rice resistance to S.cerealella. According to the difference between transgenic Bt rice and it’s control on survival rate, the number of progenies, loss rate, Dobie’s susceptible index, and other development indexes, transgenic Bt rice significantly prevents the development of S.cerealella with survival and loss rates of 13% and 12%, respectively. Dobie’s index was significantly low than control. This indicates that Bt rice grain are significantly resistant to S.cerealella.

---