Abstract:To understand the interaction of enzyme inhibitors with insecticides on Spodoptera exigua (Hübner) and Spodoptera litura (Fabricius), the laboratory toxicities of emamectin benzoate, chlorfenapyr, indoxacarb, chlorpyrifos, tebufenozide and methomyl on the 3-instars of S.exigua and S.litura were determined using topical application. The results indicated that the relative toxicities of emamectin benzoate, chlorfenapyr and indoxacarb were high on S.exigua and S.litura with the LC50 range between 0.69 μg/g and 16.32 μg/g, while only chlorpyrifos showed high toxicity to S.litura and tebufenozide to S.exigua with LC50 37.66 μg/g and 20.71 μg/g, respectively. The results of interaction of enzyme inhibitors with insecticides indicated that four different enzyme inhibitors of PBO, TPP, DEM, and SV1 showed significant restraint to chlorfenapyr and indoxacarb with the synergism ratio between 0.25 and 0.96, while showed synergism to emamectin benzoate, tebufenozide on S.exigua and emamectin benzoate, chlorpyrifos on S.litura with the synergism ratio between 1.22 and 16.2.