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期 刊 ：植物保护学报 2009年

关键词：灰飞虱；水稻品种；挥发物；行为反应；驱避性；

Keywords:Laodelphax striatellus, rice varieties, volatile, behavioral response, antixenosis,

摘 要 ：为明确镇稻2号、扬粳9538、徐稻3号及盐稻8号等4个粳稻品种驱避灰飞虱的原因,采用"H"型嗅觉仪测定灰飞虱对4个抗性品种稻株挥发物的行为反应,并通过GC-MS对挥发物的组分进行了定性和定量分析,测定其中4个组分对灰飞虱的生物活性。结果表明,灰飞虱明显偏好感虫对照武育粳3号稻株的挥发物和避开扬粳9538等抗性品种稻株的挥发物。GC-MS 分析发现,抗虫稻株挥发物的组成与感虫对照武育粳3号之间存在着明显差异。感虫对照稻株的挥发物中有38 种成分;与感虫稻株挥发物相比,徐稻3号、盐稻8号、扬粳9538、镇稻2号抗虫稻株挥发物中增加了未知20组分,且扬粳9538、镇稻2号抗虫稻株挥发物中还增加了橙花叔醇。此外,徐稻3号、扬粳9538、镇稻2号抗虫稻株挥发物中一些组分的相对含量较感虫对照中的有明显改变。生物活性测定表明,Z－法尼烯、橙花叔醇及雪松醇等3个组分对灰飞虱有明显的引诱作用。

Abstract:To clarify antixenosis and its mechanisms of 4 resistant japonica varieties including Zhendao 2, Yangjing 9538, Xudao 3 and Yandao 8 to small brown planthopper, Laodelphax striatellus Falln, behavioral responses of the small brown planthopper to volatiles emitted from resistant varieties were studied with H-shaped olfactometer. The results showed that a majority of L.striatellus were attracted to volatiles released from susceptible variety Wuyujing 3, but not to those from resistant varieties. The headspace composition of resistant rice variety was significantly different to that of Wuyujing 3, the susceptible check. Thirty-eight components were collected from headspace of Wuyujing 3. Besides the 38 components, there is an additional compound unknown 20 in volatiles from resistant varieties Xudao 3, Yandao 8, Yangjing 9538 and Zhendao 2. Nerolidol was only collected from headspace of Yangjing 9538 and Zhendao 2. Additionally, the proportions of some components in the volatiles from Xudao 3, Yangjing 9538 and Zhendao 2 varieties were different to those from susceptible check. The biological activities of 4 identified components to L.striatellus were measured with H-shaped olfactometer. The results demonstrated that L.striatellus could be attracted by (Z)- farnesene, nerolidol, and cedrol.

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