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期 刊 ：植物遗传资源学报 2015年 16卷 3期

关键词：辽宁省；主栽水稻品种；抗病基因；鉴定分析；

Keywords:Liaoning Province, Main rice cultivars, Resistance genes, Identification analysis,

摘 要 ：鉴定主栽水稻品种及育种骨干亲本的抗瘟基因型，有助于了解不同抗病基因在品种中的分布，为抗病品种选育及品种布局提供参考；本研究选取辽宁省24份水稻材料，根据7个抗稻瘟病基因的保守区设计引物，扩增各品种的编码区序列，对扩增子序列进行对比分析，鉴定了各基因在24个品种中的分布情况，并对部分抗病等位基因进行了功能验证。结果表明：辽宁省24个主栽水稻品种均不携带Pi21、Pi36、Pi37或其抗病等位基因，而Pid2、Pid3、Pita和Pik/Piks/Pikm/Pikp在24个品种中以不同突变类型及不同频率出现，其中，在2个品种中检测到Pid2抗病基因及Pid3的抗病等位基因；4个品种检测到Pita的抗病等位基因，Pita的几个等位基因中新发现到的几处碱基突变并未影响抗病基因的功能；所有品种中均无与Pik及其复等位基因完全一致的序列，但利用携带AVR-pik的稻瘟病菌接种鉴定结果表明，辽粳454和沈农265携带的Pik等位基因可能仍具有抗病基因功能。

Abstract:Identification of rice blast resistance genes in main rice cultivars and main parents help to understand the distribution of resistance genes among varieties, and provide a reference for breeding disease-resistant varieties and the makeup of varieties. In this study, 24 cultivars from Liaoning Province were used to study the distribution of resistance genes and functions of resistance alleles. Primers were designed according to the conservative region sequence of nine resistance genes to amplify the coding region of 24 cultivars. The amplification results from 24 cultivars were compared and suggested that the 24 cultivars from Liaoning Province did not carry the Pi21、Pi36、Pi37 gene or resistance alleles. However, Pid2, Pid3, Pita and Piks/Piks/Pikm/Pikp existed within the 24 varieties with different types and different mutation frequencies. Among these resistance genes, the resistance alleles Pid2 and Pid3 were detected in 24 cultivars. Pita alleles were detected in four cultivars and the newly-found base mutations did not affect resistance. The Piks/Piks/Pikm/Pikp sequence was not found in the 24 cultivars. After being inoculated with rice blast fungus with AVR-Pik, result showed that the alleles carried by varieties Liaojing454 and Shennong265 may serve resistance functions.

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